Revolutionize Your NGS Workflows

Published February 2024 | Authors Kristian Wittek, Product Manager, Morten Thorsholt, Product Manager and Huma Siddiqui, Product Manager

Laboratory technicians perform diagnostic analysis at a workstation with computers and laboratory equipmen

Unleash the Power of Precision with Tecan’s Advanced Robotic Liquid Handling Systems and Experience the Future of Sequencing with MGI’s DNBSEQ Technology.

What is DNA Extraction, and Why is it Crucial?

DNA extraction is the process of isolating DNA from the cells of various specimens, such as blood, saliva, or tissue. This pivotal step is essential in numerous scientific endeavors and investigative procedures, enabling the purified DNA to undergo further analysis. Such analyses include the identification of individuals, the diagnosis of diseases, and genetic research. Read more

Library Preperation

Library preparation is the first step of next generation sequencing. It allows DNA or cDNA to adhere to the sequencing flow cell and allows the sample to be identified.

The basic steps of library preparation are fragmentation and end repair, addition of adapters, and (optional), PCR amplification.

The MGISeq platform uses a unique DNA nanoball (DNB) technology, which involves the amplification of genomic DNA into nanoballs, followed by sequencing by synthesis (SBS) using fluorescently labeled nucleotides. Read more.

Introduction to DNBSEQ™ technology and its several advantages

MGI’s DNA sequencing instruments utilizes the state-of-the-art core technology called DNBSEQ™, a revolutionary approach to DNA sequencing. DNBs (DNB nanoballs) are pumped with the fluidics system and loaded onto patterned array chip. Sequencing primer is then added and hybridized to adaptor region of the DNB. The sequencing reaction starts by pumping sequencing reagents containing fluorescently labeled dNTP probes and DNA polymerase. Images are taken after the fluorescently labeled probes on the DNB are excited with lasers. The images are then converted into digital signal using MGI’s proprietary software. This information is then used to determine the DNA sequence of the sample.

Screen with DNA data background shows the sequence of the human genome’s

Bioinformatics

Bioinformatics combines computer science and biology to understand biological data. At Triolab, we make this complex data easy to manage and understand. Our tools, known as bioinformatics pipelines, sort through the data, analyze it, and then turn it into clear information that researchers and doctors can use. This helps with drugs discovery and customized patient treatments, ensuring our work is valuable for the future of healthcare.